Classical and Statistical Optimization of Medium Composition for Promoting Prodigiosin Produced by Local Isolate of Serratia Marcescens

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Sura Jasem Mohammed Khalid Jaber Kadhum Khalid waleed Hameed


Prodigiosin is a ‘natural red pigment produced by Serratia marcescens which exhibits immunosuppressive and anticancer properties in addition to antimicrobial activities. This work presents an attempt to maximize the production of prodigiosin by two different strategies: one factor at time (OFAT) and statistical optimization. The result of OFAT revealed that sucrose and peptone were the best carbon and nitrogen sources for pigment production with concentration of prodigiosin of about 135 mg/ L. This value was increased to 331.6mg/ L with an optimized ratio of C/N (60:40) and reached 356.8 with pH 6 and 2% inoculum size at end of classical optimization. Statistical experimental design based on Response surface methodology was conducted to optimize the composition of trace element. The design revealed that the predicted prodigiosin production of 406 mg/L can be achieved when concentrations of trace element CaCl2·2H2O, FeSO4·4H2O, MgSO4·7H2O and MnSO4·4H2O were equal to 9.22, 0.32, 0.67 and 2.48 g/L, respectively. The actual production of prodigiosin in the optimized medium was 375  mg/L. Growth kinetics of S. marcescens were evaluated in optimized medium which revealed that prodigiosin was ‘non-associated growth’ secondary metabolite with maximum production of approximately 365.7 mg/L obtained after 54 hours of incubation.


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MOHAMMED, Sura Jasem; KADHUM, Khalid Jaber; HAMEED, Khalid waleed. Classical and Statistical Optimization of Medium Composition for Promoting Prodigiosin Produced by Local Isolate of Serratia Marcescens. Al-Khwarizmi Engineering Journal, [S.l.], v. 14, n. 4, p. 92- 102, dec. 2018. ISSN 2312-0789. Available at: <>. Date accessed: 12 dec. 2018. doi:


[1] Ogba, O. M., Mandor, B. I. and Omang, H. M. (2014). Antibiotic susceptibility pattern of Serratiamarcescensisolates from woundinfections in a tertiary health institution in Calabar, Nigeria. ElMednifico Journal, 2(3), 223-226.
[2] Thomson, N. R., Crow, M. A., McGowan, S. J., Cox, A. and Salmond, G. P.C. (2000). Biosynthesis of carbapenem antibiotic and prodigiosin pigment in Serratiais under quorum sensing control.Molecular microbiology, 36(3), 539-556.
[3] Williamson, N. R., Fineran, P. C., Leeper, F. J. and Salmond, G. P. C. (2006). The biosynthesis and regulation of bacterial prodiginines. Nature Reviews Microbiology, 4(12), 887-899.
[4] Li C, Bai J, Cai Z, Ouyang F .(2001).Optimization of a cultural medium for bacteriocin production by Lactococcuslactis using response surface methodology. J Biotechnol 93:27–34. doi:10.1016/S0168-1656(01)00377-7.
[5] Chen, W.C., Yu, W.J., Chang, C.C., Chang, J.S., Huang, S.H., Chang, C.H. (2013). Enhancing production of prodigiosin from Serratiamarcescens C3 by statistical experimental design and porous carrier additionstrategy. Biochemical Engineering Journal, 78, 93-100.
[6] Gassara, F., S. K. Brar, R. D. Tyagi, R. P. John, M. Verma, and J. R. Valero (2011) Parameter optimization for production of ligninolytic enzymes using agro-industrial wastes by response surface method. Biotechnol.Bioproc. Eng. 16: 343-351.
[7] Myers R, Montgomery RC (2002) Response surface methodology: Process and product optimization using designed experiments. Wiley, New York.
[8] Venil, C. K. and Lakshmanaperumalsamy, P.(2009). Application of statistical design to the optimization of culture medium for prodigiosin production by SerratiamarcescensSWML08. Malaysian Journal of Microbiology, 5(1) 55-61.
[9] Magel, E. (1991): “Qualitative and quantitave determination of starch by colorimetric method”, Starch/Staercke, 43(10), 384-387.
[10] Wang, N. S. (2017). Sucrose assay by dinitrosalicylic colorimetric method. University of Maryland College Park, MD 207422111 ENCH485.
[11] Fender, J. E., Bender, C. M., Stella, N. A., Lahr, R. M., Kalivoda, E. J. and Shanks, R. M. Q. (2012).Serratiamarcescens quinoprotein glucose dehydrogenase activity mediates medium acidification and inhibition of prodigiosin production by glucose. Applied and environmental microbiology, 78(17), 6225-6235.
[12] Aharonowitz, Y. (1980). Nitrogen metabolite regulation of antibiotic biosynthesis. Annul Reviews Microbiology 34, 209-233.
[13] Martin J. F. and Mcdaneil ,L. E. (1977) production of polyene microlide antibiotics Adv. Appl. Microbiol. 21, 1-52.
[14] Wei,Y.H.,Chen,W.C.(2005)Enhanced production of prodigiosin-like pigment from Serratiamarcescens SM(R by medium improvement and oil-supplementation strategies, J. Biosci. Bioeng.99 ,616–622.
[15] Rokem, J. S., & Weitzman, P. (1987).Prodigiosin formation by Serratiamarcescens in a chemostat. Enzyme and Microbial Technology, 9, 153–155.
[16] WeiY.H.,ChenW.C., HuangC.K., WuH.S., SunY.M., LoC.W. andJanarthananO.M., Screening and evaluation of polyhydroxybutyrate-producing strains fromindigenous isolate Cupriavidustaiwanensis strains, Int. J. Mol. Sci. 12 (2011) 252–265.
[17] Egli, T. and Zinn, M. (2003). The concept of multiple-nutrient-limited growth of microorganisms and its application in biotechnological processes. Biotechnology advances, 22(1), 35-43.
[18] Mahmoud, S .T., Luti, K .J .K , Yonis R. W.(2015). Enhancement of prodigiosin production by SerratiamarcescensS23 via introducing microbial elicitor cells into culture medium. Iraqi Journal of Science, 56, (3A), pp: 1938-1951.
[19] Kim, D., Kim, J. F., Yim, J. H., Kwon, S.-K., Lee, C. H. and Lee, H. K. (2008). Red to red-the marine bacterium Hahellachejuensisand its product prodigiosin for mitigation of harmful algal blooms.Journal of microbiology and biotechnology, 18(10), 1621-1629.
[20] [20] Dutka, M., Ditaranto,M., and Løvås, T.(2015). Application of a Central Composite Design for the Study of NOx Emission Performance of a Low NOx Burner. Energies, 8, 3606-3627; doi:10.3390/en8053606.
[21] Osorio NM, Ferreira-Dias S, Gusmao JH, Fonseca MMR (2001) Response surfacemodeling of the production of v-3 polyunsaturated fatty acids-enriched fats by a commercial immobilized lipase. J MolCatal B Enzym 11:677–686. doi:10.1016/S1381-1177(00)00156-9.
[22] Dey G, Mitra A, Banerjee R, Maiti BR (2001) Enhanced production of amylase by optimization of nutritional constituents using response surface methodology. BiochemEng J 7:227–231. doi:10.1016/S1369 703X(00)00139-X
[23] Luti K. J. K. and Mavituna, F.(2011). Elicitation of Streptomyces coelicolor with dead cells of Bacillus subtilis and Staphylococcus aureus in a bioreactor increases production of undecylprodigiosin. Biotechnological product and process engineering. 90:461–466.